30th NARECOM – 15/11/2023, 2:30 p.m.

The presentation "The effect of metal nanoparticles on gene expression of mouse mesenchymal stem cells" will be introduced by Dr. Michal Šíma from IEM CAS, Czech Republic.

The 30th NARECOM will take place on Wednesday, November 15, 2023, at 2:30. p.m.

Join Zoom Meeting: https://cesnet.zoom.us/j/94850158062


Several metal nanoparticles (NPs) are known for their antimicrobial properties. However, they may negatively impact human organism, including mesenchymal stem cells (MSCs), a cell population contributing to tissue growth and regeneration. For the first time, we performed a comprehensive evaluation of the toxic effects of selected NPs (Ag, ZnO, and CuO) in mouse MSCs.

In this project, multiple endpoints such as reactive oxygen species production, lipid peroxidation, DNA alterations, and cell cycle were analyzed. Our results indicated the negative biological impacts of these NPs on MSC. The tested NPs induced intracellular ROS generation, which was most likely partly eliminated by the antioxidant mechanisms, resulting in limited effects on oxidative DNA damage. Furthermore, these NPs affected the G1 and S phases of the cell cycle after 24 h exposure.

We also searched for the changes in mRNA and miRNA expression caused by the exposure of mouse stem cells to the same metal nanoparticles in three various doses. After RNA isolation from cell lysates, mRNA and miRNA libraries were prepared, sequenced, and differential expression was analyzed.

Exposure of MSCs to NPs leads to expression changes most pronounced in case of silver NPs with a stronger effect on mRNA than on miRNA. The weakest reaction was observed after the treatment with zinc oxide NPs. In conclusion, the metal antimicrobial NPs tested in our study exert a negative response in MSC and might not be optimal for combined wound treatment with MSC.

Acknowledgements: This work was supported by the Czech Science Foundation (21-17720S).

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